Cell Disruption by Nitrogen Decompression
A rapid and effective way to:
- Homogenize cells and tissues
- Release intact organelles
- Prepare cell membranes
- Release labile biochemicals
- Produce uniform and repeatable homogenates without subjecting the sample to extreme chemical or physical stress.
How it Works
The principle of the method is quite simple. Large quantities of nitrogen are first dissolved in the cell under high pressure within a suitable pressure vessel. Then, when the gas pressure is suddenly released, the nitrogen comes out of the solution as expanding bubbles that stretch the membranes of each cell until they rupture and release the contents of the cell.
Parr Cell Disruption Vessels
Parr stainless steel vessels for processing cell suspensions by the nitrogen decompression method are made in several sizes with full opening heads and self sealing closures which can be handled easily on any laboratory bench without special tools or fixtures. Each vessel has two valves and a pressure gage: one valve for charging with nitrogen and the other for withdrawing the homogenate and discharging it through an attached delivery tube. All of the fittings as well as the vessel itself are made of stainless steel with polished surfaces for good corrosion resistance and freedom from contamination. The individual parts are easily cleaned and can be thoroughly sterilized.